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KMID : 0370220080520010012
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Yakhak Hoeji
2008 Volume.52 No. 1 p.12 ~ p.19
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HPLC Analysis of Organic Acids, Phenol, and Benzopyrene in Wood Vinegar
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Kim Ah-Ram
Cho Jae-Soo
Lee Gye-Won
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Abstract
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The organic acids (formic acid, acetic acid and propionic acid), phenol and benzopyrene in wood vinegar were determine by HPLC. An Atlantis™dC18 column with a acetonitrile: 0.1% phosphoric acid (5: 95, organic acids), acetonitrile: water (10 : 90, phenol), 100% acetonitrile (benzopyrene) as a mobile phase was used. Retention time of acetic acid, formic acid, propionic acid, phenol and benzopyrene was 4.77, 3.73, 9.08, 30.97 and 6.10 min, respectively. The calibration curves of organic acids, phenol, benzopyrene were linear over the concentration range of 30~500, 60~1000 , and 3~50 μg/ml with correlation coefficient of above 0.999. The limit of detection (LOD) and limit of quantitation (LOQ) of acetic acid, formic acid, propionic acid, phenol, and benzopyrene was 1.71 and 5.19, 1.11 and 3.35, 4.87 and 14.74, 6.45 and 19.55, 0.08 and 0.24 μg/ml, respectively. The coefficients of variation for intra- and inter-day assay were 0.21~4.14 and 0.07~1.19%, respectiv-ely and the precision was 95%~115%. From this study, we suggest that HPLC is suitable method for the determination of organics in wood vinegar and could be applied to quality control of wood vinegar.
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KEYWORD
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HPLC:Organic acids:Phenol, Benzopyrene, Wood vinegar
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